关键词: 模糊支持向量机, 隶属度, 紧密度, 剪接位点识别, 选择性剪接

Abstract: In order to improve the splice site recognition accuracy of Fuzzy Support Vector Machine (FSVM), a new method for computing the membership degree of sample was proposed. The initial membership was defined as the distance ratio of the sample to the two cluster centers of positive and negative samples, K-Nearest Neighbor (KNN) was adopted to compute the tightness of the samples, and the multiplication of the tightness and the initial membership degree was used as the ultimate membership. It will not only improve the membership degree of support vector, but also reduce the membership degree of noise sample. This method was applied to recognize the splice site, and the experimental results show that the recognition accuracy of constitutive 5′ and 3′ splice site reaches 94.65% and 88.97% respectively. Compared with the classical support vector machine，the recognition accuracy of constitutive 3′ splice site increases by 7.94%.

Key words: Fuzzy Support Vector Machine (FSVM), membership degree, tightness, splice site recognition, alternative splice