计算机应用 ›› 2011, Vol. 31 ›› Issue (07): 1815-1817.DOI: 10.3724/SP.J.1087.2011.01815

• 图形图像技术 • 上一篇    下一篇

基于小波分析的显微视觉景深扩展

宋宇1,2,李庆玲2,3   

  1. 1. 北京交通大学 电子信息工程学院, 北京100044
    2. 哈尔滨工业大学 机器人技术与系统国家重点实验室, 哈尔滨 150080
    3. 中国科学院 自动化研究所, 北京100190
  • 收稿日期:2010-12-07 修回日期:2011-01-23 发布日期:2011-07-01 出版日期:2011-07-01
  • 通讯作者: 宋宇
  • 作者简介:宋宇(1979-), 男, 辽宁沈阳人,讲师, 主要研究方向: 计算机视觉; 李庆玲(1979-), 女, 四川仁寿人,助理研究员, 主要研究方向: 康复机器人、假肢控制。
  • 基金资助:

    国家自然科学基金资助项目;机器人技术与系统国家重点实验室开放基金资助项目;中国博士后科学基金特别资助项目;中央高校基本科研业务费资助项目;北京交通大学人才基金资助项目

Focus depth extension for microscopic visual system based on wavelet analysis

Yu SONG1,2,Qing-ling LI2,3   

  1. 1. School of Electronic and Information Engineering, Beijing Jiaotong University, Beijing 100044, China
    2. State Key Laboratory of Robotics and System, Harbin Institute of Technology, Harbin Heilongjiang 150080, China
    3. Institute of Automation, Chinese Academy of Sciences, Beijing 100190, China
  • Received:2010-12-07 Revised:2011-01-23 Online:2011-07-01 Published:2011-07-01
  • Contact: Yu SONG
  • Supported by:

    ;State Key Laboratory of Robotics and System of HIT;Postdoctoral Special Science Foundation of China;Beijing Jiaotong University

摘要: 光学显微视觉系统的主要特点在于景深短,难以获取反映显微场景的全面信息。为解决该问题,提出基于小波分析的光学显微视觉系统景深扩展策略,分为局部清晰图像获取和多聚焦图像融合两个阶段:首先,以定义的小波系数活性水平为依据,构造了新型清晰度评价函数和聚焦曲线全局极值搜索策略来实现快速自动聚焦、获取局部显微场景信息的目的;然后,为实现多局部聚焦显微图像的融合,设计了小波系数活性水平选择型融合规则来融合获取的多个局部显微场景信息。实验表明,提出方法可有效扩展光学显微镜的景深。

关键词: 小波分析, 显微视觉, 自动聚焦, 多聚焦图像融合, 景深

Abstract: The main characteristic of optical microscopic visual is a short depth of focus, so it is difficult to obtain overall microscopic scene information. To overcome the problem, a wavelet analysis based strategy was proposed. It consists of two parts: the local information acquisition and the global information fusion. Firstly, based on the designed wavelet activity level measurement, a robust auto-focusing algorithm with new image sharpness function and fast global maximum searching strategy were designed to acquire local microscopic visual information. Secondly, an image wavelet activity level measurement based multi-focus images fusion scheme was presented. At last, the experimental results verify the validity of the proposed strategy.

Key words: wavelet analysis, microscopic vision, auto-focusing, multi-focus image fusion, focus depth

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