计算机应用 ›› 2010, Vol. 30 ›› Issue (4): 1114-1116.
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叶丙刚1,汪德鹏2,李京湘2,周妍2,吴效明2
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摘要： 边合成边测序的高通量基因测序技术存在一个相位问题，即碱基合成的先后顺序会出现“超前”和“延迟”。针对此问题，结合Markov过程的方法提出一种回归分析，在建立有关相位“超前”与“延迟”问题的荧光强度数据基础上，构造出相位校正矩阵，解决高通量基因测序中的相位问题。实验结果表明，采用提出的相位校正方法，达到对碱基合成时出现的相位“超前”和“延迟”问题的校正。
关键词: 高通量, 测序, 相位校正, Markov过程
Abstract: The high-throughput genome sequencing has a phase problem, i.e. "exceeding" or "delaying", which appears in the order of bases synthesizing. Combined with Markov process, a method of regression analysis was proposed. Based on the constructed data of fluorescence intensity, being correlated with phase "exceeding" and "delaying" questions, a phase emendation matrix was set up to resolve the phase problem. The experimental result shows the method can eliminate the phase "exceeding" or "delaying".
Key words: high-throughput, sequencing, phase emendation, Markov process
叶丙刚 汪德鹏 李京湘 周妍 吴效明. 高通量基因测序相位问题的校正研究[J]. 计算机应用, 2010, 30(4): 1114-1116.
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http://www.joca.cn/CN/Y2010/V30/I4/1114
