To improve the accuracy of focusd regions in multifocus image fusion based on multiscale transform, a multifocus image fusion algorithm was proposed based on NonSubsampled Shearlet Transform (NSST) and focused regions detection. Firstly, the initial fused image was acquired by the fusion algorithm based on NSST. Secondly, the initial focusd regions were obtained through comparing the initial fused image and the source multifocus images. And then, the morphological opening and closing was used to correct the initial focusd regions. Finally, the fused image was acquired by the Improved Pulse Coupled Neural Network (IPCNN) in the corrected focusd regions. The experimental results show that, compared with the classic image fusion algorithms based on wavelet or Shearlet, and the current popular algorithms based on NSST and Pulse Coupled Neural Network (PCNN), objective evaluation criterions including Mutual Information (MI), spatial frequency and transferred edge information of the proposed method are improved obviously. The result illustrates that the proposed method can identify the focusd regions of source images more accurately and extract more sharpness information of source images to fusion image.