关键词: 线粒体图像, 多方向模板, 追踪, 定量, 特征提取

Abstract: In order to achieve fast and accurate quantification on the feature of mitochondrial structure, a new method based on multi-directional template response and tracing algorithm was presented. Firstly, the seed points were selected from the original mitochondrial images. Edge points and centerline points of mitochondria structure were detected using directional template response on 16 directions. Then, it initiated from the seed points, and automatically traced the mitochondrial structure recursively by detecting the edge points. The number, average length and average area of mitochondria were calculated from the tracing process. The experiments show that mitochondrial morphology is significantly different (P<0.05) between controls and smooth muscle cells with overloaded free cholesterol. The proposed approach is more efficient and more accurate than the interactive method.

Key words: mitochondrial images, multi-directional templates, tracing, quantification, feature extraction